The Effects of Berberine in the Expression Quantities of Efflux Pump Genes hef'ABC of Helicobacter Pylori of Multi-drug resistance
Zhou Fan1, Xu Xiaoqing2, Peng Junwei3, Cao Qin2
1. Gastroenterology Department,The Third People’s Hospital of Huzhou,Huzhou,Zhejiang 313000,China; 2. Gastroenterology Department,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China; 3. Department of integrated Chinese and Western medicine,Shanghai Minhang District Tumor Hospital,Shanghai 220240,China
Abstract：Objective To study the the role of berberine（BBR） in the regulation of efflux pump genes hefABC in multi-drug resistant（MDR） strains of Helicobacter pylori（Hp）. Methods ①Hp clinical isolates were tested the minimum inhibitory concentration for amoxicillin,levofloxacin,clarithromycin and metronidazole by E-test method.,in which sensitive strains and MDR strains were screened.②The expression quantities diffenrence of efflux pump genes hefABC between MDR group and sensitive group were detected by Real-time fluorescent quantitative polymerase chain reaction（RT-PCR）.③The MIC values of berberine against MDR strains were determined by agar two-fold dilution method.After the MDR strains were cultured at 1/2 MIC concentration of berberine in liquid for 5 generations,RT-PCR was used to detect the mRNA expression difference of the efflux pump genes before and after the addition of berberine. Results ①A total of sixty Hp strains were isolated and screened including fifteen sensitive strains and twelve MDR strains.②The expression quantities of hefA、hefB in the MDR group are significantly higher than that in the sensitive group（P<0.05）.There are no high expressions of hefC between two groups（P<0.05）.③The expression quantities of Hp0605,Hp0606 of MDR group after the effects of berberine remarkably decrease,compared with that before treated with berberine,and the difference has statistical significance. Conclusions ①Multi-drug resistance of H.pylori is related to the high expression of efflux pump genes of Hp0605,Hp0606.②Berberine can inhibit drug resistance in some MDR strains by down-regulating the expression of efflux pump genes hefA and hefB.
周帆, 徐小青, 彭君伟, 曹勤. 黄连素对幽门螺杆菌多重耐药株hefABC基因表达的影响[J]. 哈尔滨医药, 2024, 44(4): 1-4.
Zhou Fan, Xu Xiaoqing, Peng Junwei, Cao Qin. The Effects of Berberine in the Expression Quantities of Efflux Pump Genes hef'ABC of Helicobacter Pylori of Multi-drug resistance. journal1, 2024, 44(4): 1-4.
[1] MILLER A K,WILLIAMS S M.Helicobacter pylori infection causes both protective and deleterious effects in human health and disease[J].Gennes Immun,2021,22(4):218-226. [2] 刘文忠,谢勇,陆红,等.第五次全国幽门螺杆菌感染处理共识报告[J].胃肠病学,2017,22(06): 346-360. [3] MATSUMOTO H,SHIOTANI A,GRAHAMD Y.Current and future treatment of helicobacter pylori infection-s[J].Adv Exp Med Biol,2019,1149:211-225. [4] 侯宠,刘一品.幽门螺杆菌抗菌药物耐药分子机制的研究进展[J].胃肠病学,2021,26(03): 171-175. [5] Du D,Wang-Kan X,Neuberger A,et al.Multidrug efflux pumps:Structure,function and regulation[J].Nature Re views Microbiology,2018,16(9):523-539. [6] BACHIR M,ALLEMR,BENEJAT L,et al.Molecular detection of mutations involved inHelicobacter pyloriantibiotic resistance in Algeria[J].J Antimicrob Chemother,2018,73(8) :2034-2038. [7] BEHESHTIROUY S,EYVAZI S,TARHRIZ V.Evaluation of mutations in 23S rRNA,rdxA and frxA genes of Helicobacter pylori in paraffin-embedded gastricbiopsy specimens from Iranian gastric cancer and gastritis patients[J].J Gastrointest Cancer,2021,52(1):207-211. [8] Thai KM,Ngo TD,Phan TV,et al.Virtual screening for novel Staphylococcus Aureus NorA efflux pump inhibitors from natural products[J].Med Chem,2015,11(2):135-155. [9] Chetri S,Bhowmik D,Paul D,et al.AcrAB-TolC efflux pump system plays a role in carbapenem non-susc -eptibility in Escherichiacoli[J].BMC Microbiol,2019,19(1):210. [10] 童静,李昌平.外排泵系统与幽门螺杆菌抗生素耐药性的研究进展[J].现代临床医学,2022,48(06):461-464. [11] 王琼,吴芳草,郭长城,等.以Real-time PCR方法检测耐药幽门螺杆菌中hefABC基因的表达[J].基因组学与应用生物学,2019,38(04):1797-1803. [12] 赵晶,戴金峰,黄宣,等.幽门螺杆菌耐药性及其与外排泵基因hefABC相关性[J].中华医院感染学杂志,2020,30(21):3233-3237. [13] 陈元旺. 细菌AcrAB-TolC外排泵在幽门螺杆菌耐药中的作用及机制[D].南昌:南昌大学,2014. [14] 张石磊,翟向和,王春光,等.应用高通量实时荧光定量PCR方法检测小檗碱对多药耐药大肠杆菌耐药基因的影响[J].中国兽医学报,2020,40(09):1737-1745. [15] 刘三侠. 小檗碱提取物抑制多重耐药大肠埃希菌外排喹诺酮类药物的研究[D].重庆:西南大学,2013. [16] 顾觉奋. 外排泵抑制剂研究进展[J].国外医药(抗生素分册),2020,41(01):1-10.
2024, Vol. 44 
