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| Nurr1对于LPS刺激下BV2细胞神经性炎症以及铁死亡相关基因表达的作用 |
| 范全洋, 肖晶晶, 陈晓, 朱国强 |
| 南阳医学高等专科学校第一附属医院重症医学科,河南 南阳473000 |
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摘要 目的 探讨核受体相关蛋白1（nuclear receptor related 1 protein,Nurr1）在脂多糖（lipopolysaccharide,LPS）诱导BV2细胞神经炎症损伤中的表达变化及其与炎症反应和铁死亡相关分子变化之间的关系。方法 通过LPS诱导BV2细胞构建神经炎症模型。实验分为正常对照组、模型组及Nurr1激动剂组。模型组用100 ng/mL LPS处理24h,Nurr1激动剂组在LPS处理之前先用C-DIM12（10 umol/L）预处理1h,接着再加入LPS进行进一步处理24h。以Real-time荧光定量PCR（qPCR）来检测Nurr1、白细胞介素-1β（interleukin-1β,IL-1β）及谷胱甘肽过氧化物酶4（Glutathione peroxidase 4,GPX4）mRNA的表达情况;以酶联免疫吸附测定法（ELISA）检测细胞上清中的IL-1β释放量。进行Western blot检测NLRP3以及GPX4蛋白的含量变化,用免疫荧光检测Nurr1表达情况。结果 与对照组相比,模型组Nurr1和GPX4 mRNA水平明显下降,IL-1β mRNA水平明显增加,细胞上清IL-1β释放量明显增加,NLRP3蛋白含量上升,GPX4蛋白含量减少,免疫荧光结果显示Nurr1表达减弱。与模型组相比,Nurr1激动剂组Nurr1和GPX4 mRNA水平增高,IL-1β mRNA水平下降,IL-1β释放减少,NLRP3蛋白水平下降,GPX4蛋白水平上升。结论 Nurr1在LPS刺激下的BV2细胞神经炎症损伤中呈低表达水平。激活Nurr1可以缓解炎症反应,同时能调节NLRP3/GPX4等分子失调的现象的发生,证明了Nurr1可能参与调节着神经炎症以及铁死亡相关信号通路的过程。
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| 关键词 ：
Nurr1,
神经炎症,
铁死亡,
BV2细胞,
qPCR,
Western blot,
免疫荧光
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收稿日期: 2026-04-11
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通讯作者:
肖晶晶
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2026, Vol. 46 
