Toxic Effects of Shikonin in Different Concentrations on Cultured Rat Cardiomyocytes
Chen Juying1, Huang Wendong2, Gao Qi3, Lian Xiaoshan1
1. Department of Pharmacy,Guangzhou Taihe Cancer Hospital,Guangzhou 510030,China; 2. Department of Pharmacy,Maoming People's Hospital,Maoming 525099,China; 3. Department of Obstetrics and Gynecology,Guangzhou Women's and Children's Medical Center,Guangzhou 510600,China
Abstract：Objective To explore the toxic effects of rat cardiomyocytes treated with different concentrations of shikonin. Methods Select neonatal rats born from 1 to 3 births,and culture the cardiomyocytes of their ventricles.and the cardiomyocytes were treated with shikonin in different concentration gradients（1×10-7mol/L,1×10-6mol/L,1×10-5mol/L）by MTT method for 48h,Cytotoxicity after 72h. Results Using MTT test results,it was found that the cytotoxicity test effect was 48h,72h,and when the shikonin concentration was 10-5mol/L,the cardiomyocytes had significant toxicity（P<0.05）,and the shikonin concentration was 1×10-7mol/L.At L,1×10-6mol/L,cardiomyocytes had no significant toxicity（P>0.05）. Conclusions hikonin has certain cytotoxicity.When the concentration of shikonin is less than 1×10-6mol/L,it does not show obvious toxicity.When the concentration of shikonin is 10-5mol/L,the cardiomyocytes are treated for 48h and 72h.Rat cardiomyocytes have obvious toxicity.
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2022, Vol. 42 
