|
|
|
| Effect of Matrine on Proliferation Invasion and TGF-β1/Smad4 Protein Expression to colon cancer cell line SW620 |
| Wan Liyan1, Wang Hao1, Jiang Hanwen2, Chen Xiaohua3, Chen Shuilian1, Chen Zhishan1, Yu Qian1, Tian Ying1, Tong Qiang4 |
1. Department of Pathology,Guangzhou Panyu Central Hospital,Guangzhou 511400,China;
2. Department of Pathology,Guangzhou Panyu maternal and Child Health Hospital,Guangzhou 511402,China;
3. Cancer Center,Integrated Hospital of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510220;
4. Department of Pathology,Guangzhou Daan Clinical Laboratory Center,Guɑnɡzhou 510665,China |
|
|
|
|
Abstract Objective To investigate the effect of matrine on proliferation,invasion and TGF-β1,Smad4 protein expression in colon cancer cell line SW620. Methods The effect of matrine on proliferative activity in SW620 cells were evaluated by CCK8 assay and colony formation assay.The effect of matrine on migration and invasion ability in SW620 cells were evaluated by Transwell assay.Quantitative analysis the effect of matrine on Ki-67,E-cadherin,TGFβ1 and Smad4 proteins expression in SW620 cell blocks by Immunohistochemistry. Results The proliferation and invasion of SW620 cells were significantly weakened after matrine treatment.TGF-β1 and Smad4 proteins expression in SW620 cells were absent,matrine had no significant effect on the proteins expression of TGF-β1 and Smad4. Conclusion Matrine can inhibit the proliferation and metastasis of SW620 cells,but the TGF-β1/Smad4 signaling pathway mechanism may be independent.
|
|
Received: 05 May 2023
|
|
|
|
|
|
[1] Biller LH,Schrag D.Diagnosis and treatment of metastatic colorectal cancer:a review[J].JAMA,2021,325(7):669-685.
[2] 张玺. 苦参碱抗肿瘤作用分子机制研究[D].华中科技大学,2020.
[3] 刘桐,刘佳,逯欣欣.苦参碱对人结肠癌HT-29细胞增殖、凋亡与侵袭的作用及机制研究[J].解剖科学进展,2020,26(3):292-294+298.
[4] 李金州,陈娜娜,戴美琴,等.苦参碱抑制结肠癌细胞生长和迁移侵袭的作用研究[J].中药材,2017,40(9):2161-2165.
[5] 李楠静,张又.苦参碱对人结肠癌SW620细胞增殖及RhoA基因表达的影响[J].四川医学,2015,36(4):481-484.
[6] 万里燕. EZH2在肺癌中表达的病理学意义分析[D].南方医科大学,2012.
[7] 申洪. 免疫组化染色定量方法研究(Ⅲ)[J].中国组织化学与细胞化学杂志,1995,4(1):89.
[8] 张梁,黄涵,黄永秩,等.TGF-β1和Smad4在大肠癌中的表达及其临床意义[J].右江医学,2010,38(1):10-11.
[9] Zhang T,Wang XF,Wang ZC,et al.Current potential therapeutic strategies targeting the TGF-β/Smad signaling pathway to attenuate keloid and hypertrophic scar formation[J].Biomed Pharmacother,2020,129(2):110287.
[10] Frangogiannis N.Transforming growth factor-β in tissue fibrosis.J Exp Med,2020,217(3):e20190103.
[11] 刘岩青,盛吉章,赵磊,等.Smad4在结肠癌组织中的表达及其临床意义[J].安徽医药,2016,20(7):1329-1333.
[12] Jiang Z,Cao Q,Dai G,et al.Celastrol inhibits colorectal cancer through TGF-β1/Smad signaling[J].Onco Targets Ther,2019,12:509-518.
[13] Yan W,Liu Z,Yang W,et al.miRNA expression profiles in Smad4-positive and Smad4-negative SW620 human colon cancer cells detected by next-generation small RNA sequencing[J].Cancer Manag Res,2018,10:5479-5490.
[14] 王菁雯,沈克平,胡兵.中医药对大肠癌信号通路的调控作用[J].中国实验方剂学杂志,2018,24(1):227-234.
[15] 邹瞭南,莫德龙,陈国滨,等.苦参碱对K-ras基因突变型结肠癌细胞株增殖的抑制作用及机制研究[J].广州中医药大学学报,2016,33(5):703-709.
[16] 杨鹏,张松,郭舜,等.苦参碱抑制Akt信号通路诱导结肠癌SW480细胞凋亡[J].中国实验方剂学杂志,2017,23(7):109-113. |
|
|
|
2023, Vol. 43 
