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| Overexpression of Chemokine Receptor 2 Combined with Rat Adipose Derived Stem Cells(ADSCs)Promotes Wound Healing of Full-thickness Skin Defects |
| Ren Pengli, Wang Lu* |
| Department of Medical Cosmetology,Second Affiliated Hospital of Zhengzhou University,Henan,Zhengzhou,450000 |
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Abstract Objective To investigate the effect of rat adipose-derived stem cells(ADSCs)overexpressing chemokine receptor CXCR2 on the healing of skin defects. Methods Three SD rats were executed,the adipose tissue around the groin and epididymis was rapidly isolated,and ADSCs were extracted by enzymatic digestion and cultured to the third generation.The morphological characteristics of the cells were examined by inverted microscopy,while flow cytometric analysis was used to assess the phenotypic markers of the cells and their differentiation ability.Forty mice were selected to construct an animal model of dorsal total skin defects and randomly assigned to four experimental groups:control group(given saline treatment),adipose-derived stem cells(ADSCs)treatment group,CXCR2 intervention group,and combined treatment group(ADSCs and CXCR2 co-application).Saline,ADSCs suspension(100 μL PBS),or CXCR2(10μg/L)were injected locally at the edge and base of the trauma,respectively.The healing progress was monitored by calculating the percentage of wound area to the initial area and the tissue changes of the wounds were observed by HE staining;the serum levels of IL-1β,IL-18 and TNF-α were detected by ELISA.The serum levels of IL-1β,IL-18 and TNF-α were detected by ELISA.The data between groups were compared by ANOVA and t-test. Results Under the microscope,primary ADSCs initially appeared spindle-shaped,transitioning to circular after 2 hours,and predominantly spindle-shaped by 48 hours.The third generation of cells exhibited a vortex arrangement.The combination therapy group showed significantly accelerated wound healing compared to the model group,with healing areas notably reduced at days 3,7,14and 21(P<0.01).HE staining indicated complete wound healing and minimal inflammatory cell infiltration in the combination group,while the ADSCs and CXCR2 groups showed incomplete healing with persistent inflammatory cells and granulation tissue.The model group exhibited slow healing and marked scar tissue hyperplasia.The serum levels of IL-1β,IL-18and TNF-α in the combination group were significantly lower than in the model group(P<0.01). Conclusion Overexpression of CXCR2 and ADSCs significantly accelerated skin wound healing and reduced inflammation.
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Received: 22 April 2025
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[1] 陈祎琦,周莹芊,魏茜,等.负载人脐带间充质干细胞来源的小细胞外囊泡的甲基丙烯酸酐化明胶水凝胶治疗小鼠全层皮肤缺损创面的效果[J].中华烧伤与创面修复杂志,2024,40(04):323-332.
[2] 范永梅,谷涓华,曾跃勤,等.虾青素纳米乳液对大鼠全层皮肤缺损创面的愈合和愈后瘢痕增生的影响及其作用机制研究[J].中国药学杂志,2023,58(15):1376-1383.
[3] 池凯,毋磊,陈龙金,等.人脱细胞真皮复合骨髓间充质干细胞构建组织工程皮肤修复皮肤创面缺损[J].中国组织工程研究,2018,22(26):5.
[4] 金剑,储云高.海藻糖凝胶对大鼠全层皮肤缺损创面及兔耳瘢痕增生的影响[J].中华烧伤与创面修复杂志,2024,40(07):679-688.
[5] 余逸凡,刘科杰,麦麦提图尔荪,等.脂肪干细胞治疗类风湿性关节炎的研究进展[J].中国当代医药,2024(15).
[6] 林美玉,姚翔,高景,等.年轻和老年小鼠脂肪来源干细胞生物学特性对比[J].中国组织工程研究,2025,29(19):4063-4068.
[7] 张春连,孙振龙,王璐.人脂肪干细胞与表皮生长因子促进皮肤创面愈合的研究[J].中华实验外科杂志,2021,38(9):6.
[8] 陶紫涵. 二甲双胍提高脂肪干细胞促血管化能力的研究[D].中国人民解放军海军军医大学,2024.
[9] 马蕊. 负载P物质和脂肪干细胞的甲基丙烯酰化明胶-丝素蛋白水凝胶促进皮肤创口愈合的研究[D].吉林大学,2024.
[10] 付文,王向臣,王延桂,等.脂肪源性间充质干细胞外泌体在大鼠全层皮肤缺损创面愈合中的机制研究[J].组织工程与重建外科,2023,19(4):342-351.
[11] Jürgen Becker,Schwoch S,Zelent C,et al.Transcriptome Analysis of Hypoxic Lymphatic Endothelial Cells Indi-cates Their Potential to Contribute to Extracellular Ma-trix Rearrangement[J].Cells,2021,10(5):1008-1023.
[12] 丁泓帆,徐潇,陈敏亮,等.ADSCs-exo对小鼠树突状表皮T细胞及皮肤创面炎症反应调控的影响[J].中国美容整形外科杂志,2024,35(06):363-368.
[13] 刘大诚,李艺楠.PRP联合ADSCs负载藻酸钙凝胶修复软骨缺损的效果研究[J].局解手术学杂志,2024,33(05):393-398.
[14] 赖仲宏. 淫羊藿苷对脂肪干细胞成软骨分化的影响及其相关机制研究[D].赣南医学院,2023.
[15] 白鹏,韩桂珍,鲁丽.支气管哮喘患儿痰液中CXCR2、CCR1、CCL3、CCL2表达及与肺功能和气道炎症的关系[J].河北医药,2019,41(23):3555-3558. |
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2025, Vol. 45 
