To Investigate the Effect of Inorganic Selenium on Metabolic Bone Disease in Rats
Zhao Xingjiao1, Zhu Zeyu2, Zhang Yongfang3,*
1. Hubei Minzu University,The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,China; 2. Pediatrics Department,Zhuzhou Central Hospital,Zhuzhou 412007,China; 3. Child Health and Rehabilitation Psychiatry Department,The Central Hospital OF ENSHI Tujia and Miao Autonomous Prefecture,Enshi 445000,China
Abstract：Objective To explore the effects of different selenium levels on metabolic bone disease in rats and observe the response after vitamin D treatment. Methods Rats were randomly divided into a blank control group,a non selenium supplemented group,a low selenium group,and a high selenium group.There were 30 rats in the blank control group,32 rats in the non selenium supplemented group,42 rats in the low selenium group,and 42 rats in the high selenium group.Except for the blank control group,the other three groups of rats were modeled using hydroxyethylenediphosphonic acid.After 7 days of subcutaneous injection,8 rats were randomly selected to collect samples for modeling and vitamin D testing.The successful modeling was confirmed,and then the rats were fed in groups.The low selenium group was given inorganic selenium [1.0mg/(kg·w) sodium selenite] by gavage,the high selenium group was given inorganic selenium [5.0mg/(kg·w)sodium selenite] by gavage,and the non selenium group was also given an equal amount of physiological saline by gavage.After 4 weeks of continuous collection,samples were collected to test serum bone metabolism biochemical indicators; Administer a one-time intramuscular injection of 9000 U/100g of vitamin D,continue the above dose of selenium gavage for 4 weeks,and collect samples to test serum bone metabolism biochemical indicators. Results When supplementing with selenium alone,there was no significant increase in serum 25 hydroxyvitamin D levels in the experimental group rats,and there was no significant difference between the groups.However,the alkaline phosphatase in the high selenium group was lower than that in the non supplemented and low selenium groups(P<0.05);After selenium combined with vitamin D supplementation treatment,the serum levels of 25 hydroxyvitamin D in the experimental group rats were significantly increased compared to before,and the low selenium group was higher than the non selenium group(P<0.05). Conclusion Low selenium can promote the conversion of vitamin D to 25 hydroxyvitamin D in a rat model of metabolic bone disease.
[1] ALBAHRI A S,ALWAN J K,TAHA Z K,et al.IoT-based telemedicine for disease prevention and health promotion:State-of-the-Art[J].Journal of Network and Computer Applications,2020. [2] CHINOY A,MUGHAL M Z,PADIDELA R.Metabolic bone disease of prematurity:causes,recognition,prevention,treat-ment and long-term consequences[J].Archives of Disease in Childhood-Fetal and Neonatal Edition,2019. [3] 刘国荣. 早产儿代谢性骨病相关危险因素的临床研究[D].2019. [4] UKARAPONG S,VENKATARAYAPPA S K B,NAVARR ETE C,et al.Risk factors of metabolic bone disease of prematurity[J].Early Human Development,2017. [5] 陈文昊,杨长仪,张宝泉.197例新生儿骨折临床分析[J].福建医科大学学报,2015,49(02):123-6. [6] 张迪,何娜,杨晓莉,等.硒蛋白对人体健康重要作用的研究进展[J].科学通报,2022,67(06):473-80. [7] EBERT R,ULMER M,ZECK S,et al.Selenium supple-mentation restores the antioxidative capacity and prevents cell damage in bone marrow stromal cells in vitro[J].Science Signaling,2006. [8] GILBERT A K,NEWTON T D,HETTIARATCHI M H,et al.Reactive sulfur and selenium species in the regulation of bone homeostasis[J].Free Radical Biology and Medicine,2022. [9] CAO J J,GREGOIRE B R,ZENG H.Selenium deficiency decreases antioxidative capacity and is detrimental to bone microarchitecture in mice[J].J Nutr,2012,142(8):1526-31. [10] EBERT R,JAKOB F.Selenium deficiency as a putative risk factor for osteoporosis[J].International Congress Series,2007,1297:158-64. [11] MORENO-REYES R,MATHIEU F,BOELAERT M,et al.Selenium and iodine supplementation of rural Tibetan children affected by Kashin-Beck osteoarthropathy[J].Am J Clin Nutr,2003,78(1):137-44. [12] MORENO-REYES R,EGRISE D,NèVE J,et al.Selenium deficiency-induced growth retardation is associated with an impaired bone metabolism and osteopenia[J].J Bone Miner Res,2001,16(8):1556-63. [13] GAFFNEY-STOMBERG E.The Impact of Trace Minerals on Bone Metabolism[J].Biol Trace Elem Res,2019,188(1):26-34. [14] TEITELBAUM S L,ROSS F P.Genetic regulation of oste-oclast development and function[J].Nat Rev Genet,2003,4(8):638-49. [15] YANG T,LEE S Y,PARK K C,et al.The Effects of Selenium on Bone Health:From Element to Therapeutics[J].Molecules,2022,27(2). [16] DENG H,LIU H,YANG Z,et al.Progress of Selenium Deficiency in the Pathogenesis of Arthropathies and Selenium Supplement for Their Treatment[J].Biol Trace Elem Res,2022,200(10):4238-49. [17] LUK K-H,CHAN C-H,LIU Z-W,et al.Selenium na-noparticles functionalized by mushroom polysaccharide-protein complex:A novel nano-mineral for managing post-menopausal osteoporosis[J].Journal of Functional Foods,2023,110:105832. [18] MANOLAGAS S C.From estrogen-centric to aging and oxidative stress:a revised perspective of the pathogenesis of osteoporosis[J].Endocr Rev,2010,31(3):266-300. [19] WAUQUIER F,LEOTOING L,COXAM V,et al.Oxidative stress in bone remodelling and disease[J].Trends Mol Med,2009,15(10):468-77. [20] ZHOU Q,CHEN W,GU C,et al.Selenium-modified bone cement promotes osteoporotic bone defect repair in ov-ariectomized rats by restoring GPx1-mediated mitochon-drial antioxidant functions[J].Regen BiomaterSelenium-modified bone cement promotes osteoporotic bone defect repair in ov-ariectomized rats by restoring GPx1-mediated mitochon-drial antioxidant functions[J].Regen Biomater,2023,10:rbad-011. [21] TANG X,MA S,LI Y,et al.Evaluating the Activity of Sodium Butyrate to Prevent Osteoporosis in Rats by Pro-moting Osteal GSK-3β/Nrf2 Signaling and Mitochon-drial Function[J].J Agric Food Chem,2020,68(24):6588-603. [22] BA Y,SUN L,ZUO J,et al.Association of oxidative stress and Kashin Beck disease integrated Meta and Bioin-formatics analysis[J].Osteoarthritis and Cartilage,2022. [23] ZHANG Z,ZHANG J,XIAO J.Selenoproteins and selen-ium status in bone physiology and pathology[J].Biochim Biophys Acta,2014,1840(11):3246-56. [24] HU W,YAO X,LI Y,et al.Injectable hydrogel with sel-enium nanoparticles delivery for sustained glutathione peroxidase activation and enhanced osteoarthritis the-rapeutics[J].Materials Today Bio,2023. [25] JAKOB F,BECKER K,PAAR E,et al.Expression and re-gulation of thioredoxin reductases and other selenopr-oteins in bone[J].Methods Enzymol,2002,347:168-79. [26] SHARMA U,PAL D,PRASAD R.Alkaline Phosphatase:An Overview[J].Indian Journal of Clinical Biochemistry,2014,29(3):269-278. [27] GEORGIOS K,SOTIRIA D,OLGA-ELPIS K,et al.The Role of Bone Alkaline Phosphatase and Osteocalcin in Saliva as Indicators of Skeletal Maturity in Children[J].Applied Sciences,2023. [28] CHAN G M,ARMSTRONG C,MOYER-MILEUR L,et al.Growth and bone mineralization in children born prematurely[J].Journal of Perinatology,2008,28(9):619-623. [29] ZIERK J,ARZIDEH F,HAECKEL R,et al.Pediatric re-ference intervals for alkaline phosphatase[J].Clin Chem Lab Med,2017,55(1):102-110. [30] BIERLA K,SZPUNAR J,LOBINSKI R,et al.Effect of graded levels of selenium supplementation as selenite on expression of selenosugars,selenocysteine,and other selenometabolites in rat liver[J].Metallomics,2023,15(11). [31] PAPP L V,LU J,HOLMGREN A,et al.From selenium to selenoproteins:synthesis,identity,and their role in human health[J].Antioxid Redox Signal,2007,9(7):775-806. [32] KRYSIAK R,KOWALCZE K,OKOPIE? B.Selenomethio-nine potentiates the impact of vitamin D on thyroid autoimmunity in euthyroid women with Hashimoto's thy-roiditis and low vitamin D status[J].Pharmacol Rep,2019,71(2):367-373. [33] XU W M,HE J,YU M Q,et al.Site-Selective modi-fication of vitamin D analogue (Deltanoid) through a resin-based version of organoselenium 2,3-sigmatropic rearrangement[J].Org Lett,2010,12(19):4431-4433. [34] BRIGELIUS-FLOHé R,FLOHé L.Regulatory Phenomena in the Glutathione Peroxidase Superfamily[J].Antioxid Redox Signal,2020,33(7):498-516. [35] KUDVA A K,SHAY A E,PRABHU K S.Selenium and inflammatory bowel disease[J].Am J Physiol Gastrointest Liver Physiol,2015,309(2):G71-7.
2025, Vol. 45 
