NLK Promotes the Proliferation,Migration,Invasion,and EMT of Bladder Cancer Cells by Regulating Paxillin
Lu Jianjun1, Yu Qin2, Chen Lixiong1,*
1. Department of Urology,the Thired People's Hospital,Yuhang District,Hangzhou,Zhejiang 311115,China; 2. Department of Urology,Beilun District People's Hospital,Ningbo 315000,China
Abstract：Objective This research was intended to exploring the effect and mechanism of kinase（NLK）and paxillin on proliferation,migration,invasion,and epithelial mesenchymal transition（EMT）of bladder cancer cells,and providing theoretical basis for basic research,clinical prevention and treatment of bladder cancer. Methods The expression of NLK in bladder cancer tissue was analyzed by bioinformatics analysis.The proliferation,migration and invasion of bladder cancer cells were detected by EdU staining assay,clone formation assay,and transwell assay.RNA pull down assay was used to detect the interaction between NLK and paxillin in bladder cancer cells.Western blot assay was used to detect the expressions of NLK,p-paxillin,and EMT-related proteins in bladder cancer cells. Results NLK and p-paxillin were highly expressed in bladder cancer;NLK interacted with paxillin;NLK downregulation inhibited the proliferation,migration,invasion,EMT,and p-paxillin expression of bladder cancer cells;paxillin overexpression reversed the effect of silencing NLK on proliferation,migration,invasion,EMT,and p-paxillin expression in bladder cancer cells. Conclusion NLK promoted the proliferation,migration,invasion and EMT of bladder cancer cells by regulating paxillin,providing theoretical basis for basic research,clinical prevention and treatment of bladder cancer.
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2024, Vol. 44 
