DNA微阵列芯片法在非结核分枝杆菌菌种鉴定的应用探讨

doi:10.3969/j.issn.1001-8131.2024.01.008

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摘要目的为临床工作中非结核分枝杆菌（NTM）的感染和防治提供科学依据。方法收集疑似结核分枝杆菌感染患者标本（包括呼吸道标本痰液、灌洗液以及非呼吸道标本无菌体液）共1089例,采用DNA微阵列芯片法进行检测,分析分枝杆菌菌种的分布特点。结果 1089份标本中检出结核分枝杆菌878份（80.62%）,NTM211份（19.38%）。其中非结核分枝杆菌菌种分布有5种,胞内分枝杆菌153例（72.51%）,鸟分枝杆菌28例（13.27%）,龟脓分枝杆菌复合群19例（9%）,堪萨斯分枝杆菌10例（4.74%）,耻垢分枝杆菌1例（0.48%）。非结核分枝杆菌感染人员分布男性127例（60.19%）,女性84例（39.81%）,年龄分布以老年为主,60岁以上103例（48.82%）,男性71例（68.93%）,女性32例（31.07%）。结论疑似结核分枝杆菌患者检出NTM共有5种,排名前三的NTM种类是胞内分枝杆菌、鸟分枝杆菌和龟脓分枝杆菌复合群,感染人群以老年男性为主。

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	谭蛟
	王亚春
	王亚丽
	夏爽
	程美锦
	王伟

关键词 ：非结核分枝杆菌, 菌种鉴定, DNA微阵列芯片技术

Abstract：Objective To investigate the isolation of non-tuberculous mycobacterium（NTM）in Henan Provincial Chest Hospital,and to provide a scientific basis for the infection and prevention of NTM in clinical work in this region. Methods 1089 duplicate specimens（including sputum,lavage solution and sterile bodily fluids of non-respiratory specimens）of suspected mycobacterium tuberculosis infection in Henan Chest Hospital were detected by DNA microarray chip method,and the distribution characteristics of Mycobacterium species were analyzed. Results In 1089 specimens,878（80.62%）of Mycobacterium tuberculosis were detected,and 211（19.38%）were detected in NTM.Among them,there were 5 species of non-tuberculous Mycobacterium species,153 cases（72.51%）were detected in Intracellular Mycobacteria,28 cases（13.27%）of Mycobacterium avium ornithus,19 cases（9%）of Mycobacterium purpureum complex,10 cases（4.74%）of Mycobacterium Kansas,and 1 case（0.48%）of Mycobacterium diureum.Among the 211 specimens,127 males（60.19%）,84 female patients（39.81%）,and the age distribution was mainly elderly,103 cases over 60 years old（48.82%）,including 71 male patients（68.93%）and 32 female patients（31.07%）;Conclusions 5 kinds of NTM were detected in patients with suspected Mycobacterium tuberculosis admitted to Henan Chest Hospital,and the top three NTM species were Mycobacterium intracellular,Mycobacterium avium and Mycobacterium turtle complex,and the infected population was mainly elderly men.

Key words： Non-mycobacterium tuberculosis Strain identification DNA microarray chip technology

收稿日期: 2023-01-16

PACS:

R378

通讯作者: 王伟

引用本文:

谭蛟, 王亚春, 王亚丽, 夏爽, 程美锦, 王伟. DNA微阵列芯片法在非结核分枝杆菌菌种鉴定的应用探讨[J]. 哈尔滨医药, 2024, 44(1): 26-28.
Tan Jiao, Wang Yachun, Wang Yali, Xia Shuang, Cheng Meijin, Wang Wei. Application of DNA Microarray Chip Method in the Identification of Non-Tuberculous Mycobacterium Species. journal1, 2024, 44(1): 26-28.

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[1] 中华医学会结核病学分会.非结核分枝杆菌病诊断与治疗指南(2020年版)[J].中华结核和呼吸杂志,2020,43(11):918-946.
[2] 唐神结,高文.临床结核病学[M].2版.北京:人民卫生出版社,2019:1026-1046.
[3] Daley CL,Iaccarino JM,Lange C,et al.Treatment of nontuberculous mycobacterial pulmonary disease:an official ATS/ERS/ESCMID/IDSA clinical practice guideline[J].Eur Respir J,2020,56(1):200053.DOI:org/10.1183/ 13993003.00535-2020.
[4] Haworth CS,Banks J,Capstick T,,et al.British Thoracic Society guidelines for the management of non tuberculous mycobacterial pulmonary disease.British Thoracic Society guidelines for the management of non tuberculous mycobacterial pulmonary disease(NTM PD)[J].Thorax,2017,72(Suppl 2):ii1 ii64.DOI:10.1136/thoraxjnl 2017 210927.
[5] 刘珑玲,陈世玖.非结核分枝杆菌感染的分子生物学快速诊断技术[J].中华医学杂志,2014,94(38):3039-3040.
[6] 王黎霞,成诗明,陈明亭,等.2010年全国第五次结核病流行病学抽样调查报告[J].中国防痨杂志,2012,34(08):485-508.
[7] 陈涛,许琳,杨慧娟,等.云南省非结核分枝杆菌流行状况及病原谱分析[J].中国热带医学,2018,18(09):863-865.DOI:10.13604/j.cnki.46-1064/r.2018.09.01.
[8] 杨新宇,赵琰枫,易俊莉,等.北京市结防机构2019年非结核分枝杆菌临床分离株危险因素及耐药情况分析[J].临床肺科杂志,2022,27(08):1148-1153.
[9] 杨新宇,张洁,易俊莉,等.2009和2019年北京市非结核分枝杆菌流行特征比较[J].首都公共卫生,2021,15(06):333.
[10] 张洁,苏建荣,丁北川,等.北京地区非结核分枝杆菌菌种分布及耐药性研究[J].中华结核和呼吸杂志,2017,40(03):210-214.
[11] 洪创跃,李金莉,赵广录,等.2013-2017年深圳市非结核分枝杆菌流行状况分析[J].中国防痨杂志,2019,41(05):529-533.
[12] 李文彬,胡培磊,陈忠南,等.湖南省525株非结核分枝杆菌临床分离株的菌种鉴定与流行特征[J].中国人兽共患病学报,2022,38(05):417-422.
[13] 陈忠南,易松林,胡培磊,等.2012-2017年湖南省非结核分枝杆菌感染的特征分析[J].中国防痨杂志,2019,41(02):217-221.
[14] 余斐,陈晓,嵇仲康,等.杭州地区2009-2014年非结核分枝杆菌流行状况分析[J].中国微生态学杂志,2016,28(07):808-810+815.DOI:10.13381/j.cnki.cjm.201607015.
[15] 雷杰,吴玲,王楠,等.广州地区552株非结核分枝杆菌感染患者流行病学分析[J].现代医院,2021,21(08):1245-1247+1251.