<em>ALDH1A1</em>和miR-140：宫颈癌的潜在治疗靶点和策略

doi:10.3969/j.issn.1001-8131.2025.03.001

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Abstract Objective This study aimed to explore the expression levels of ALDH1A1 and miR-140 in cervical cancer cell lines and study their interactions.Through in-depth understanding of the mechanism of action of ALDH1A1 and miR-140 in the occurrence and development of cervical cancer,it provides new potential targets and treatment strategies for the diagnosis and treatment of cervical cancer. Methods Cervical cancer dataset was obtained from the NCBI GEO database,and R（3.5.0）software package Limma was used to screen Differentially Expressed Genes（DEGs）on the chip data.The selected differentially expressed genes were analyzed by Gene Ontology Biological Process（GO BP）function and Kyoto Encyclopedia of Genes and Genomes（KEGG）pathway,to construct the competing endogenous RNA network（ceRNA）of differential mRNA,microRNA and lncRNA;the protein-protein interaction network（PPI）of differential mRNA was constructed to select Hub genes.The Kaplan-Meier survival curve was drawn to understand its risk of disease,and cell experiments and qRT-PCR were performed to detect the expression levels of differential mRNA and target miRNA in each group of cells,and double luciferase experiments were performed to verify the interaction between the two. Results By analyzing the cervical cancer dataset in NCBI GEO database,the Hub gene ALDH1A1 was screened out and its miRNA was found.From the Kaplan-Meier curve,it was concluded that ALDH1A1 was a risk factor for the occurrence and development of cervical cancer,and the prognosis of the high expression group was significantly worse than that of the low expression group.The results of cell experiments showed that in cervical cancer cell lines,the expression level of ALDH1A1 was higher,while the expression level of miR-140 was lower.Further experiments showed that miR-140 could significantly inhibit the expression of ALDH1A1 in cervical cancer cell lines. Conclusion The high expression of ALDH1A1 and the low expression of miR-140 may be closely related to the occurrence and development of cervical cancer.miR-140 targets ALDH1A1 and inhibits its expression.This reveals the important role of ALDH1A1 and miR-140 in the occurrence and development of cervical cancer.

Key words： Cervical cancer ALDH1A1 miR-140

Received: 26 February 2025

PACS:

R711.74

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http://www.hrbyybjb.org.cn/EN/10.3969/j.issn.1001-8131.2025.03.001 OR http://www.hrbyybjb.org.cn/EN/Y2025/V45/I3/1

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