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| Study on Tanshinone ⅡA Inducing Differentiation of CD44+CD24-/low Breast Cancer Stem Cells |
| Sun Hongguang1, Wang Yanming2, Jia Zhongming1 |
1. Department of Breast Surgery,Affiliated Hospital of Binzhou Medical University,Binzhou,Shandong 256603,China;
2. Binzhou Medical University,Binzhou, 256603,China |
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Abstract Objective To isolate the CD44+CD24-/low subpopuLation from breast cancer cells using MACS immunomagnetic bead sorting,and to investigate the differentiation-inducing effect of tanshinoneⅡA on this cell subpopuLation. Methods The CD44+CD24-/low cell subpopuLation was sorted by MACS immunomagnetic bead sorting,and the proportion of this subpopuLation before and after sorting was determined by flow cytometry.After co-cuLturing the CD44+CD24-/low cell subpopuLation with different concentrations of tanshinoneⅡA,the cell cycle distribution was detected by flow cytometry to clarify the role of tanshinoneⅡA in the differentiation of breast cancer stem cells. Results Approximately 6% of CD44+CD24-/low cells were successfuLly obtained via MACS immunomagnetic bead sorting,and flow cytometric identification showed that the purity of this cell subpopuLation reached 93.85%.After intervention with different concentrations of tanshinone ⅡA,the distribution ratios of CD44+CD24-/low cells in G0/G1 phase,S phase and G2/M phase exhibited significant differences.As the concentration of tanshinone ⅡA increased from 0 μg/mL to 10 μg/mL,the proportion of cells in G0/G1 phase decreased continuously from 92.83±0.25% to 84.07±0.15%,indicating that tanshinoneⅡA exerted a remarkable concentration-dependent reguLatory effect on cell cycle progression. Conclusion The CD44+CD24-/low cell subpopuLation can be successfuLly isolated using MACS immunomagnetic bead sorting.TanshinoneⅡA can promote more cells of this subpopuLation to exit the quiescent G0/G1 phase and enter the active proliferation cycle,accelerate the processes of DNA replication(S phase)and cell division(G2/M phase),thereby enhancing cell proliferative activity.
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Received: 04 February 2026
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2026, Vol. 46 
