不同浓度紫草素对培养大鼠心肌细胞的毒性作用分析

doi:10.3969/j.issn.1001-8131.2022.04.002

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Abstract Objective To explore the toxic effects of rat cardiomyocytes treated with different concentrations of shikonin. Methods Select neonatal rats born from 1 to 3 births,and culture the cardiomyocytes of their ventricles.and the cardiomyocytes were treated with shikonin in different concentration gradients（1×10^-7mol/L,1×10^-6mol/L,1×10^-5mol/L）by MTT method for 48h,Cytotoxicity after 72h. Results Using MTT test results,it was found that the cytotoxicity test effect was 48h,72h,and when the shikonin concentration was 10^-5mol/L,the cardiomyocytes had significant toxicity（P<0.05）,and the shikonin concentration was 1×10^-7mol/L.At L,1×10^-6mol/L,cardiomyocytes had no significant toxicity（P>0.05）. Conclusions hikonin has certain cytotoxicity.When the concentration of shikonin is less than 1×10^-6mol/L,it does not show obvious toxicity.When the concentration of shikonin is 10^-5mol/L,the cardiomyocytes are treated for 48h and 72h.Rat cardiomyocytes have obvious toxicity.

Key words： Shikonin Cardiomyocytes Toxic effect

Received: 31 December 2021

PACS:

R944

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	Articles by authors
	Chen Juying
	Huang Wendong
	Gao Qi
	Lian Xiaoshan

Cite this article:

Chen Juying,Huang Wendong,Gao Qi, et al. Toxic Effects of Shikonin in Different Concentrations on Cultured Rat Cardiomyocytes[J]. journal1, 2022, 42(4): 3-5.

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http://www.hrbyybjb.org.cn/EN/10.3969/j.issn.1001-8131.2022.04.002 OR http://www.hrbyybjb.org.cn/EN/Y2022/V42/I4/3

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