|
|
|
| Role of Mycobacterium Tuberculosis DNA Detection in Paraffin Tissue in Tuberculosis Screening |
| Chen Huiqin1, Zheng Jie2,* |
1. Pathology Department of Xinglin Hospital(Department of Pathology, Xianglin Branch of the First Affiliated Hospital of Xiamen University), Fujian Xiamen361022, China;
2. Department of Pathology, First Affiliated Hospital of Xiamen University, Fujian Xiamen, 361000, China |
|
|
|
|
Abstract Objective To analyze the clinical value of real-time fluorescent quantitative PCR(FQ-PCR) for detection of Mycobacterium tuberculosis(MTB) DNA in paraffin tissue in screening tuberculosis(TB). Method 118 TB patients admitted to our hospital from January 2017 to December 2019 were selected,and tissue specimens were collected,embedded in paraffin,and subjected to pathological examination;Furthermore,FQ-PCR and acid fast staining of tissue sections were used to detect MTB-DNA,and the positive detection rates of the three methods were compared. Result Among the paraffin embedded tissues of 118 selected patients,FQ-PCR,acid fast staining,and histopathological methods were used to detect MTB positivity in 70,50,and 65 cases,respectively,with positive rates of 59.32%,42.37%,and 55.08%,respectively.The positive rates of the three methods were significantly different(P<0.05).Compared with the acid fast staining method,the positive detection rate of MTB in lung,pleural,lymph node biopsy,and other tissue specimens is higher,FQ-PCR、 The histopathological method showed higher detection rates(P<0.05),while the positive detection rate of FQ-PCR in lung biopsy specimens was higher than that of histopathological method(P<0.05). Conclusion The use of FQ-PCR for detecting MTB-DNA in paraffin tissue is more effective than acid fast staining and tissue pathology methods in lung biopsy.It can be used as an important tool for screening TB and provide support for tissue pathology diagnosis.
|
|
Received: 31 March 2026
|
|
|
|
|
|
[1] Burki T.WHO's 2024 global hepatitis report[J].Lancet Infect Dis,2024,24(6):e362-e363.
[2] 李政,方乐天,胡明,等.2006-2021年中国居民结核病死亡情况的流行特征分析与预测[J].中华流行病学杂志,2024,45(6):824-832.
[3] 李姗姗,王玉峰,舒薇,等.结核病实验室诊断技术研发新进展[J].中国防痨杂志,2023,45(5):446-453.
[4] 孙天文,王晓艳.荧光定量PCR检测结核分枝杆菌DNA与涂片抗酸染色在肺结核诊断中应用比较[J].深圳中西医结合杂志,2023,33(16):62-64.
[5] 张梦迪,王鑫,郑文静,等.老年结核病危险因素研究进展[J].中国防痨杂志,2024,46(10):1266-1272.
[6] 李中实,王自立.我国结核病诊疗的进展及现状[J].中国脊柱脊髓杂志,2021,31(7):577-578.
[7] 刘宁,付洪义,孙金昊,等.四种病原学方法在检测痰液标本中结核杆菌的诊断价值对比[J].公共卫生与预防医学,2021,32(6):107-110.
[8] 杜威,刘春雨,王路生,等.结核病发病机制的研究进展[J].中国人兽共患病学报,2022,38(3):217-225.
[9] 刘晨,钱影臣,左安欣,等.结核分枝杆菌检测的三种方法学评价[J].诊断病理学杂志,2024,31(12):1226-1228.
[10] 张晓坤,李勰璘,李筱慧.三种肺结核实验室检测的方法学评价和临床应用价值[J].广东医学,2024,45(3):382-385. |
| [1] |
. [J]. journal1, 2026, 46(4): 48-51. |
| [2] |
. [J]. journal1, 2026, 46(3): 52-55. |
| [3] |
Zhong Bing, Jiao Jie, Li Fangfei, Lin Biding. Comparative Observation of the Clinical Efficacy of the Same Scheme in the Treatment of Simple Pulmonary Tuberculosis and Pulmonary Tuberculosis with Diabetes[J]. journal1, 2025, 45(5): 9-11. |
| [4] |
. [J]. journal1, 2025, 45(4): 71-73. |
| [5] |
. [J]. journal1, 2023, 43(4): 119-121. |
| [6] |
. [J]. journal1, 2023, 43(3): 85-87. |
|
|
|
|
2026, Vol. 46 
