黄连素对幽门螺杆菌多重耐药株<em>hefABC</em>基因表达的影响

doi:10.3969/j.issn.1001-8131.2024.04.001

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Abstract Objective To study the the role of berberine（BBR） in the regulation of efflux pump genes hefABC in multi-drug resistant（MDR） strains of Helicobacter pylori（Hp）. Methods ①Hp clinical isolates were tested the minimum inhibitory concentration for amoxicillin,levofloxacin,clarithromycin and metronidazole by E-test method.,in which sensitive strains and MDR strains were screened.②The expression quantities diffenrence of efflux pump genes hefABC between MDR group and sensitive group were detected by Real-time fluorescent quantitative polymerase chain reaction（RT-PCR）.③The MIC values of berberine against MDR strains were determined by agar two-fold dilution method.After the MDR strains were cultured at 1/2 MIC concentration of berberine in liquid for 5 generations,RT-PCR was used to detect the mRNA expression difference of the efflux pump genes before and after the addition of berberine. Results ①A total of sixty Hp strains were isolated and screened including fifteen sensitive strains and twelve MDR strains.②The expression quantities of hefA、hefB in the MDR group are significantly higher than that in the sensitive group（P<0.05）.There are no high expressions of hefC between two groups（P<0.05）.③The expression quantities of Hp0605,Hp0606 of MDR group after the effects of berberine remarkably decrease,compared with that before treated with berberine,and the difference has statistical significance. Conclusions ①Multi-drug resistance of H.pylori is related to the high expression of efflux pump genes of Hp0605,Hp0606.②Berberine can inhibit drug resistance in some MDR strains by down-regulating the expression of efflux pump genes hefA and hefB.

Key words： berberine helicobater pylori multi-drug resistance efflux pump gene

Received: 01 May 2024

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R573.6

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	Zhou Fan
	Xu Xiaoqing
	Peng Junwei
	Cao Qin

Cite this article:

Zhou Fan,Xu Xiaoqing,Peng Junwei, et al. The Effects of Berberine in the Expression Quantities of Efflux Pump Genes hef'ABC of Helicobacter Pylori of Multi-drug resistance[J]. journal1, 2024, 44(4): 1-4.

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http://www.hrbyybjb.org.cn/EN/10.3969/j.issn.1001-8131.2024.04.001 OR http://www.hrbyybjb.org.cn/EN/Y2024/V44/I4/1

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