DNA微阵列芯片法在非结核分枝杆菌菌种鉴定的应用探讨

doi:10.3969/j.issn.1001-8131.2024.01.008

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Abstract Objective To investigate the isolation of non-tuberculous mycobacterium（NTM）in Henan Provincial Chest Hospital,and to provide a scientific basis for the infection and prevention of NTM in clinical work in this region. Methods 1089 duplicate specimens（including sputum,lavage solution and sterile bodily fluids of non-respiratory specimens）of suspected mycobacterium tuberculosis infection in Henan Chest Hospital were detected by DNA microarray chip method,and the distribution characteristics of Mycobacterium species were analyzed. Results In 1089 specimens,878（80.62%）of Mycobacterium tuberculosis were detected,and 211（19.38%）were detected in NTM.Among them,there were 5 species of non-tuberculous Mycobacterium species,153 cases（72.51%）were detected in Intracellular Mycobacteria,28 cases（13.27%）of Mycobacterium avium ornithus,19 cases（9%）of Mycobacterium purpureum complex,10 cases（4.74%）of Mycobacterium Kansas,and 1 case（0.48%）of Mycobacterium diureum.Among the 211 specimens,127 males（60.19%）,84 female patients（39.81%）,and the age distribution was mainly elderly,103 cases over 60 years old（48.82%）,including 71 male patients（68.93%）and 32 female patients（31.07%）;Conclusions 5 kinds of NTM were detected in patients with suspected Mycobacterium tuberculosis admitted to Henan Chest Hospital,and the top three NTM species were Mycobacterium intracellular,Mycobacterium avium and Mycobacterium turtle complex,and the infected population was mainly elderly men.

Key words： Non-mycobacterium tuberculosis Strain identification DNA microarray chip technology

Received: 16 January 2023

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R378

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	Articles by authors
	Tan Jiao
	Wang Yachun
	Wang Yali
	Xia Shuang
	Cheng Meijin
	Wang Wei

Cite this article:

Tan Jiao,Wang Yachun,Wang Yali, et al. Application of DNA Microarray Chip Method in the Identification of Non-Tuberculous Mycobacterium Species[J]. journal1, 2024, 44(1): 26-28.

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http://www.hrbyybjb.org.cn/EN/10.3969/j.issn.1001-8131.2024.01.008 OR http://www.hrbyybjb.org.cn/EN/Y2024/V44/I1/26

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