The Effects of Berberine in the Expression Quantities of Efflux Pump Genes hef'ABC of Helicobacter Pylori of Multi-drug resistance
Zhou Fan1, Xu Xiaoqing2, Peng Junwei3, Cao Qin2
1. Gastroenterology Department,The Third People’s Hospital of Huzhou,Huzhou,Zhejiang 313000,China; 2. Gastroenterology Department,Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China; 3. Department of integrated Chinese and Western medicine,Shanghai Minhang District Tumor Hospital,Shanghai 220240,China
AbstractObjective To study the the role of berberine（BBR） in the regulation of efflux pump genes hefABC in multi-drug resistant（MDR） strains of Helicobacter pylori（Hp）. Methods ①Hp clinical isolates were tested the minimum inhibitory concentration for amoxicillin,levofloxacin,clarithromycin and metronidazole by E-test method.,in which sensitive strains and MDR strains were screened.②The expression quantities diffenrence of efflux pump genes hefABC between MDR group and sensitive group were detected by Real-time fluorescent quantitative polymerase chain reaction（RT-PCR）.③The MIC values of berberine against MDR strains were determined by agar two-fold dilution method.After the MDR strains were cultured at 1/2 MIC concentration of berberine in liquid for 5 generations,RT-PCR was used to detect the mRNA expression difference of the efflux pump genes before and after the addition of berberine. Results ①A total of sixty Hp strains were isolated and screened including fifteen sensitive strains and twelve MDR strains.②The expression quantities of hefA、hefB in the MDR group are significantly higher than that in the sensitive group（P<0.05）.There are no high expressions of hefC between two groups（P<0.05）.③The expression quantities of Hp0605,Hp0606 of MDR group after the effects of berberine remarkably decrease,compared with that before treated with berberine,and the difference has statistical significance. Conclusions ①Multi-drug resistance of H.pylori is related to the high expression of efflux pump genes of Hp0605,Hp0606.②Berberine can inhibit drug resistance in some MDR strains by down-regulating the expression of efflux pump genes hefA and hefB.
Zhou Fan,Xu Xiaoqing,Peng Junwei, et al. The Effects of Berberine in the Expression Quantities of Efflux Pump Genes hef'ABC of Helicobacter Pylori of Multi-drug resistance[J]. journal1, 2024, 44(4): 1-4.
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2024, Vol. 44 
