子痫前期中Nrf2和NQO1的关系及NQO1对HTR-8/SVneo细胞侵袭力影响的研究

doi:10.3969/j.issn.1001-8131.2022.04.010

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Abstract Objective To investigate the relationship between nuclear factor E2 related factor 2（Nrf2）and quinone oxidoreductase-1（NQO1）in preeclampsia and the effect of NQO1 on the invasiveness of htr-8 / svneo cells. Methods 74 cases of preeclampsia and 74 cases of normal pregnant women were selected.The plasma samples were collected one day before cesarean section.The levels of Nrf2 and NQO1 were measured by enzyme-linked immunosorbent assay（ELISA）,and Pearson correlation analysis was performed.Htr-8 / svneo cells were pretreated with Nrf2 inducer（TBHQ）,NQO1 inducer（2-hbas）,Nrf2 inhibitor（ml385）and NQO1 inhibitor（dicoumarol）,respectively.Then,htr-8 / svneo cells were pretreated with hydrogen peroxide or blank treatment for 4 h.The relative expression level of NQO1 mRNA and Nrf2 and NQO1 mRNA in htr-8 / svneo cell model were detected to analyze the invasiveness of htr-8 / svneo cells. Results The levels of Nrf2 and NQO1 in preeclampsia patients were lower than those in the control group,the difference was statistically significant（P<0.05）;the expression levels of Nrf2 and NQO1 in preeclampsia patients were positively correlated（P<0.05）;compared with the control group,the relative expression levels of Nrf2 and NQO1 protein and NQO1 mRNA in cells of hydrogen peroxide group and TBHQ group were higher,and the difference was statistically significant（P<0.05）;ml385 There was no significant difference in the relative expression levels of Nrf2,NQO1 protein and NQO1 mRNA between the two groups（P > 0.05）;compared with the hydrogen peroxide group,the relative expression levels of Nrf2 and NQO1 proteins and NQO1 mRNA in the cells of the hydrogen peroxide combined with ml385 group were lower,and the difference was statistically significant（P<0.05）. Conclusion The decrease of plasma Nrf2 and NQO1 levels in patients with preeclampsia may lead to the decrease of antioxidant capacity.Under oxidative stress,Nrf2 can activate NQO1 in htr-8 / svneo cells,thus enhancing cell invasion.

Key words： Preeclampsia Quinone oxidoreductase 1 Nuclear factor E2 related factor 2 Quinone oxidoreductase 1

Received: 01 November 2021

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R714.24+5

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	Wu Guohong

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Wu Guohong. The Relationship Between Nrf2 and NQO1 in Preeclampsia and the Effect of NQO1 on the Invasiveness of htr-8 / svneo Cells[J]. journal1, 2022, 42(4): 25-27.

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http://www.hrbyybjb.org.cn/EN/10.3969/j.issn.1001-8131.2022.04.010 OR http://www.hrbyybjb.org.cn/EN/Y2022/V42/I4/25

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