基于生物信息学分析miR-152在子痫前期发病机制中的作用

doi:10.3969/j.issn.1001-8131.2023.02.001

Abstract
Figure/Table
References
Related Citation (4)

Download: PDF (468 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To analyze the differentially expressed miRNAs associated with preeclampsia,select miR-152 predicted target genes and perform bioinformatics analysis to explore the molecular mechanisms involved in the pathogenesis of preeclampsia. Methods The most significantly upregulated miR-152 in the dataset GSE103542 was selected as the study target,and its target genes were predicted and taken for intersection.GO functional annotation and KEGG pathway enrichment analysis of the intersecting target genes were performed using the DAVID network online enrichment tool,and the STRING website was used to analyze their protein interactions. Results The 418 intersecting target genes obtained are mainly involved in biological processes such as bioadhesion,cell adhesion and cell developmental regulation;in cell composition,they are mainly involved in plasma membrane components,lattice protein vesicles;in molecular functions,they are mainly involved in calcium binding,ion binding.KEGG signaling pathway enrichment analysis showed that it was mainly involved in signaling pathways regulating stem cell pluripotency and TGF signaling pathway.Protein interaction analysis showed that IGF1R,SMAD3,RPS6KB1 and PPP2CA were the key nodes of the protein interaction network. Conclusion miR-152 may affect trophoblast proliferation,invasion and apoptosis through the TGF signaling pathway in multiple ways in the development of pre-eclampsia.

Key words： preeclampsia/miR-152/target genes/bioinformatics analysis

Received: 27 April 2022

PACS:

R734.2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	Yang Qianwen
	Tao Feng
	Chen Hongbo

Cite this article:

Yang Qianwen,Tao Feng,Chen Hongbo. Bioinformatics-based Analysis of The Role of miR-152 in The Pathogenesis of Pre-eclampsia[J]. journal1, 2023, 43(2): 1-4.

URL:

http://www.hrbyybjb.org.cn/EN/10.3969/j.issn.1001-8131.2023.02.001 OR http://www.hrbyybjb.org.cn/EN/Y2023/V43/I2/1

[1] Rana S,Lemoine E,Granger JP,et al.Preeclampsia:Pathophysiology,Challenges,and Perspctives[J].CircRes,2019,124(7):1094-1112.
[2] Lv Y,Lu C,Ji X,et al.Roles of microRNAs in preeclampsia[J].J Cell Physiol,2018,234(2):1052-1061.
[3] 李娟,高敏,马琳,等.血清miR-195、miR-152的水平与子痫前期患者病情程度的关系研究及对PE发生风险的预测价值[J].中国优生与遗传杂志,2020,28(12):1463-1466.
[4] Li Q,Long A,Jiang L,et al.Quantification of preecla-mpsia-related microRNAs in maternal serum[J].Biomed Rep,2015,3(6):792-796.
[5] Huang DW,Sherman BT,Lempicki RA.Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources[J].Nature Protocols,2009,4(1):44-57.
[6] 顾蔚蓉,李笑天.子痫前期的干预与管理[J].中国实用妇科与产科杂志,2020,36(2):120-123.
[7] Jim B,Karumanchi SA.Preeclampsia:pathogenesis,prevention,and long-term complications[J].Semin Nephrol,2017,37(4):386-397.
[8] 芦德,苏放明.生物标记物预测子痫前期的研究进展[J].现代妇产科进展,2016,25(10):793-796.
[9] Philipone E,Yoon AJ,Wang S,et al.MicroRNAs-208b-3p,204-5p,129-2-3p and 3065-5p as predictive markers of oral leukoplakia that progress to cancer[J].Am J Cancer Res,2016,6(7):1537-1546.
[10] 陈世超,解丹,苏媛媛,等.MicroRNA-152在妇科生殖道恶性肿瘤中的研究进展[J].中国现代医学杂志,2021,31(1):27-32.
[11] 焦秋香,牛世坤.重度子痫前期患者血清与胎盘组织中miRNA-152和miRNA-210表达改变及其意义[J].中国优生与遗传杂志,2018,26(3):54-56.
[12] 李娟,高敏,马琳,等.血清miR-195、miR-152的表达水平与子痫前期患者病情程度的关系研究及对PE发生风险的预测价值[J].中国优生与遗传杂志,2020,28(12):1463-1466.
[13] Ansar M,Wang CJ,Wang YH,et al.SMAD3 Hypomethylation as a Biomarker for Early Prediction of Colorectal Cancer[J].Int J Mol Sci,2020,21(19):7395.
[14] Tang PM,Zhou S,Meng XM,et al.Smad3 promotes cancer progression by inhibiting E4BP4 mediated NK cell development[J].Nat Commun,2017,8:14677.
[15] Wang Y,He H,Liyanarachchi S,et al.The role of SMAD3 in the genetic predisposition to papillary thyroid carcinoma[J].Genet Med,2018,20(9):927-935.
[16] Qu X,Fang Y,Zhuang S,et al.Micro-RNA miR-542-3p suppresses decidualization by targeting ILK pathways in human endometrial stromal cells[J].Sci Rep,2021,11(1):7186.
[17] 苏冬梅,李源,马旭.Smad4在重度子痫前期患者胎盘组织中的表达及意义[J].中国妇幼保健,2014,29:2968-2970.
[18] 张红森,郭国营,杨翠等.MiR-152在膀胱癌中的表达以及对ERBB3/AKT2信号通路的调节作用[J].中国肿瘤,2019,28(1):55-62.
[19] 余敏兰,胡国潢.MiR-152-3p以TGFA为靶向调控甲状腺乳头状癌的发生[J].湖南师范大学学报(医学版),2020,17(3):35-39.
[20] Tang XL,Lin L,Song LN,et al.Hypoxia-inducible miR-152 suppresses the expression of WNT1 and ERBB3,and inhibits the proliferation of cervical cancer cells[J].Experimental Biology & Medicine,2016,241(13):1429-1437.
[21] 罗成,杨林,邹永根.miR-152通过调节AKT-ERK信号通路对骨肉瘤细胞增殖和迁移的影响[J].现代肿瘤医学,2019,27(21):3752-3756.
[22] Li LW,Xiao HQ,Ma R,et al.miR-152 is involved in the proliferation and metastasis of ovarian cancer through repression of ERBB3[J].International Journal of Molecular Medicine,2018,41(3):1529-1535.
[23] 焦秋香,牛世坤.重度子痫前期患者血清与胎盘组织中miRNA-152和miRNA-210表达改变及其意义[J].中国优生与遗传杂志,2018,26(3):54-56.
[24] 李娟,高敏,马琳,等.血清miR-195、miR-152的表达水平与子痫前期患者病情程度的关系研究及对PE发生风险的预测价值[J].中国优生与遗传杂志,2020,28(12):1463-1466.
[25] Perera M,Tsang CS,Distel RJ,et al.TGF-betal interactome:metastasis and beyond[J].Cancer Genomics Proteomics,2010,7(4):217-229.
[26] Korpal M,Kang YB.Targeting the transforming growth factor-β signalling pathway in metastatic cancer[J].Eur J Cancer,2010,46(7):1232-1240.
[27] Ansar M,Wang CJ,Wang YH,et al.SMAD3 Hypomethylation as a Biomarker for Early Prediction of Colorectal Cancer[J].Int J Mol Sci,2020,21(19):7395.
[28] Tang PM,Zhou S,Meng XM,et al.Smad3 promotes cancer progression by inhibiting E4BP4 mediated NK cell development[J].Nat Commun,2017,8:14677.
[29] Wang Y,He H,Liyanarachchi S,et al.The role of SMAD3 in the genetic predisposition to papillary thyroid carcinoma[J].Genet Med,2018,20(9):927-935.
[30] Qu X,Fang Y,Zhuang S,et al.Micro-RNA miR-542-3p suppresses decidualization by targeting ILK pathways in human endometrial stromal cells[J].Sci Rep,2021,11(1):7186.
[31] 苏冬梅,李源,马旭.Smad4在重度子痫前期患者胎盘组织中的表达及意义[J].中国妇幼保健,2014,29:2968-2970.